Journal: The Journal of International Medical Research

Article Title: Clinical application and importance of one-step human CYP2C19 genotype detection

doi: 10.1177/0300060518787718

Figure Lengend Snippet: CYP2C19 genotyping comparison between one-step qPCR genotyping, qPCR genotyping assay kit, and pyrophosphate sequencing

Article Snippet: Genotyping of the common loss-of-function CYP2C19*2 variant (rs4244285) and the CYP2C19*3 variant (rs4986893) was performed using TaqMan® single nucleotide polymorphism genotyping assays (Coyote Bioscience, Peking, China).

Techniques: Comparison, Genotyping Assay, Sequencing

Journal: The Journal of International Medical Research

Article Title: Clinical application and importance of one-step human CYP2C19 genotype detection

doi: 10.1177/0300060518787718

Figure Lengend Snippet: CYP2C19 genotyping accuracy of the one-step qPCR genotyping assay kit compared with pyrophosphate sequencing

Article Snippet: Genotyping of the common loss-of-function CYP2C19*2 variant (rs4244285) and the CYP2C19*3 variant (rs4986893) was performed using TaqMan® single nucleotide polymorphism genotyping assays (Coyote Bioscience, Peking, China).

Techniques: Genotyping Assay, Sequencing

Journal: Journal of Clinical Medicine

Article Title: Detection of BRAFV600E in Liquid Biopsy from Patients with Papillary Thyroid Cancer Is Associated with Tumor Aggressiveness and Response to Therapy

doi: 10.3390/jcm9082481

Figure Lengend Snippet: Detection of BRAFV600E by microfluidic digital PCR alone or in combination with COLD-PCR. Each panel represents a single experiment whereby DNA from BCPAP cells was segregated into individual wells and assessed for the presence of the mutant alleles using two different fluorophores (FAM and VIC). The signals from the FAM (blue) and VIC (red) dyes are plotted on the Y axis and the X axis, respectively. The yellow cluster represents the unamplified wells (negative calls). ( A ) Two-dimensional plots of microfluidic digital PCR reads out of 1 ng DNA extracted from BCPAP cells. Blue cluster represents the wells that were positive for the BRAFV600E mutation. ( B ) Results of dPCR analysis after the enrichment of BRAFV600E by COLD-PCR demonstrating 100-fold increase in mutant alleles (blue cluster). dPCR, digital Polymerase chain reaction; COLD, co-amplification at lower denaturation temperature; PCR, Polymerase chain reaction; FAM, fluorescein; VIC, 2′-chloro-7′phenyl-1,4-dichloro-6-carboxy-fluorescein.

Article Snippet: Enriched samples were used for the detection of BRAFV600E and wild-type BRAF alleles by real-time PCR using the Taqman Single-nucleotide polymorphism (SNP) Genotyping assay for BRAFV600E (Precipio, NE, USA) or for the detection of BRAFV600E and wild-type BRAF alleles using microfluidic digital PCR.

Techniques: Digital PCR, Co-amplification at Lower Denaturation temperature PCR, Mutagenesis, Polymerase Chain Reaction

Journal: Journal of Clinical Medicine

Article Title: Detection of BRAFV600E in Liquid Biopsy from Patients with Papillary Thyroid Cancer Is Associated with Tumor Aggressiveness and Response to Therapy

doi: 10.3390/jcm9082481

Figure Lengend Snippet: Clinicopathological features of the BRAFV600 -positive papillary thyroid carcinomas.

Article Snippet: Enriched samples were used for the detection of BRAFV600E and wild-type BRAF alleles by real-time PCR using the Taqman Single-nucleotide polymorphism (SNP) Genotyping assay for BRAFV600E (Precipio, NE, USA) or for the detection of BRAFV600E and wild-type BRAF alleles using microfluidic digital PCR.

Techniques: Clinical Proteomics, Biomarker Discovery, Variant Assay

Journal: Journal of Clinical Medicine

Article Title: Detection of BRAFV600E in Liquid Biopsy from Patients with Papillary Thyroid Cancer Is Associated with Tumor Aggressiveness and Response to Therapy

doi: 10.3390/jcm9082481

Figure Lengend Snippet: Risk stratification of the patients with BRAFV600 -positive papillary thyroid carcinomas.

Article Snippet: Enriched samples were used for the detection of BRAFV600E and wild-type BRAF alleles by real-time PCR using the Taqman Single-nucleotide polymorphism (SNP) Genotyping assay for BRAFV600E (Precipio, NE, USA) or for the detection of BRAFV600E and wild-type BRAF alleles using microfluidic digital PCR.

Techniques: Clinical Proteomics

Journal: Journal of Clinical Medicine

Article Title: Detection of BRAFV600E in Liquid Biopsy from Patients with Papillary Thyroid Cancer Is Associated with Tumor Aggressiveness and Response to Therapy

doi: 10.3390/jcm9082481

Figure Lengend Snippet: Response to the treatment related to risk classification and cf BRAFV600E detection.

Article Snippet: Enriched samples were used for the detection of BRAFV600E and wild-type BRAF alleles by real-time PCR using the Taqman Single-nucleotide polymorphism (SNP) Genotyping assay for BRAFV600E (Precipio, NE, USA) or for the detection of BRAFV600E and wild-type BRAF alleles using microfluidic digital PCR.

Techniques: Mutagenesis

Journal: Journal of Clinical Medicine

Article Title: Detection of BRAFV600E in Liquid Biopsy from Patients with Papillary Thyroid Cancer Is Associated with Tumor Aggressiveness and Response to Therapy

doi: 10.3390/jcm9082481

Figure Lengend Snippet: The risk factors associated with non-excellent response to treatment (either biochemical or structurally incomplete or indeterminate response).

Article Snippet: Enriched samples were used for the detection of BRAFV600E and wild-type BRAF alleles by real-time PCR using the Taqman Single-nucleotide polymorphism (SNP) Genotyping assay for BRAFV600E (Precipio, NE, USA) or for the detection of BRAFV600E and wild-type BRAF alleles using microfluidic digital PCR.

Techniques: Biomarker Discovery